abstract：:The aim of this study was to characterize further the human skin fibroblast membrane receptor for LDL. We herein report that LDL particles bound to colloidal gold in conjunction with the surface replication technique can be used to visualize steps in the movement of their receptors in the plane of the lipid bilayer an...

journal_title：European journal of cell biology

authors： Robenek H,Hesz A

更新日期：1983-09-01 00:00:00

abstract：:Isolated microtubule protein from axopodia of the heliozoan Echinosphaerium nucleofilum, consisting of two major bands on SDS-polyacrylamide gel electrophoresis (SDS-PAGE), has been compared to axonemal and cytoplasmic tubulins from both animal and non-animal sources. The upper E. nucleofilum protein band migrated fas...

journal_title：European journal of cell biology

authors： Little M,Quinlan RA,Hoffman EJ,Ludueña RF

更新日期：1983-07-01 00:00:00

abstract：:Changes in the surface morphology of regenerating rabbit, rat and frog corneal endothelial cells in vivo have been investigated by scanning electron microscopy. In adult tissue these cells do not normally divide unless given a stimulus, such as injury. Surfaces of quiescent rabbit and rat cells are devoid of microvill...

journal_title：European journal of cell biology

authors： Gordon SR,Rothstein H,Harding CV

更新日期：1983-07-01 00:00:00

abstract：:It was previously shown that cultured mouse peritoneal macrophages ingest anionic derivatives of horseradish peroxidase (HRP) and ferritin by fluid-phase endocytosis and accumulate them in lysosomes. Cationic derivatives were taken up by adsorptive endocytosis and transported to lysosomes but subsequently appeared als...

journal_title：European journal of cell biology

authors： Stenseth K,Hedin U,Thyberg J

更新日期：1983-07-01 00:00:00

abstract：:Actin, myosin, and the actin-associated proteins tropomyosin, alpha-actinin, vinculin, and villin were localized in acinar cells of rat and bovine pancreas, parotid, and prostate glands by means of immunofluorescent staining of both frozen tissue sections and semithin sections of quick-frozen, freeze-dried, and plasti...

journal_title：European journal of cell biology

authors： Drenckhahn D,Mannherz HG

更新日期：1983-05-01 00:00:00

abstract：:Dictyosomes, Golgi vesicles, and plasma membranes were investigated after freeze-fracture in cells from growing root tips of cress (Lepidium sativum L.), that are distinguishable by different cellulose content of the cell wall, into (i) meristematic cells during early formation of the cell plate, (ii) statocytes of th...

journal_title：European journal of cell biology

authors： Volkmann D

更新日期：1983-05-01 00:00:00

abstract：:By double indirect immunofluorescence and interference electron microscopy, we have observed the effect of microtubule disruption by antimitotic drugs and coldness treatment on the distribution of adhesion sites and of the three cytoskeletal proteins, vinculin, alpha-actinin, and actin in normal rat cells and in rat c...

journal_title：European journal of cell biology

authors： David-Pfeuty T

更新日期：1983-03-01 00:00:00

abstract：:In the oil containing spores of Bryum capillare, lipid breakdown occurs within three days after sowing. The periods of activity of acid, neutral and alkaline lipases are coincident. They start at the first day after sowing the spores. After 3 days of germination, when storage fats forming large globules are mobilised ...

journal_title：European journal of cell biology

authors： Carrapiço F,Salomé M,Pais S

更新日期：1983-03-01 00:00:00

abstract：:Ultrastructural studies of the digestive system of Paramecium caudatum focusing on the first 5 min of digestive-vacuole age reveal a set of vesicles, named phagosome fusion vesicles (PFVs), which fuse with the digestive vacuole just after the vacuoles are released from the cytopharynx and concomitant with vacuole acid...

journal_title：European journal of cell biology

authors： Allen RD,Fok AK

更新日期：1983-01-01 00:00:00

abstract：:Ovary gland cells of Aptenia cordifolia were exposed to 100 micrograms/ml cytochalasin B (cyt B) for 30 or 60 min during the phase of granulocrine polysaccharide secretion. The drug caused a congestion of Golgi vesicles around the dictyosomes, probably resulting from an inhibition of the vesicle migration towards the ...

journal_title：European journal of cell biology

authors： Kristen U,Lockhausen J

更新日期：1983-01-01 00:00:00

abstract：:Na/K-ATPase of salt-stressed salt glands of the domestic duck (Anas platyrhynchos) was purified in membrane-bound form by incubation of the microsomal fraction with sodium dodecylsulphate and ATP followed by discontinuous sucrose gradient centrifugation. Gel electrophoresis of the purified plasma membrane preparation ...

journal_title：European journal of cell biology

authors： Gassner D,Komnick H

更新日期：1983-01-01 00:00:00

abstract：:Cytochrome b5 has been visualized by an immunoferritin labeling in a Golgi preparation. When the Golgi apparatus was first disrupted before labeling, 60% of the profiles were clearly labeled with ferritin. This proportion of labeled vesicles was too high to be explained by contamination from other membranes. When the ...

journal_title：European journal of cell biology

authors： Collot M,Kalff M,Remacle J

更新日期：1982-11-01 00:00:00

abstract：:The method for electron microscopy of the Ag-staining of NOR-specific proteins was adapted to tissue sections from solid organs. The distribution of the Ag-staining proteins in rat liver nucleoli after complete block of transcription caused by D-galactosamine was investigated. In control animals, the Ag-staining prote...

journal_title：European journal of cell biology

authors： Dimova RN,Markov DV,Gajdardjieva KC,Dabeva MD,Hadjiolov AA

更新日期：1982-10-01 00:00:00

abstract：:The membrane characteristics of amphioxus myotome cells were investigated using the chopped-current clamp method. The extremely thin myotome lamellae had a membrane resistance of 2.2 to 7.7 k omega cm2 and a capacity of 0.4 to 1.3 microF/micron2. The steady state current-voltage relation showed outward rectification. ...

journal_title：European journal of cell biology

authors： Melzer W

更新日期：1982-10-01 00:00:00

abstract：:A zymogen granule fraction has been isolated from rat pancreas, and its purity has been assessed by biochemical and morphological criteria. Specific activities of two marker enzymes, amylase and chymotrypsin, are increased by 4.6 and 5.4-fold, respectively, as compared to the homogenate. The purified fraction is devoi...

journal_title：European journal of cell biology

authors： Pâquet MR,St-Jean P,Roberge M,Beaudoin AR

更新日期：1982-08-01 00:00:00

abstract：:Tetrahymena pyriformis GL is a suitable model to study the influence on phagocytotic activity of two membrane-stabilizing benzimidazole nitrogen mustard derivative, ZIMET 3164 and ZIMET 3393 (cytostasan). Treatment by both compounds causes a gradual inhibition of food vacuole formation dependent on the concentrations ...

journal_title：European journal of cell biology

authors： Brutkowska M,Fritsch RS,Wollweber L

更新日期：1982-08-01 00:00:00

abstract：:A three-dimensional cytoskeleton, morphologically composed of intermediate filaments, desmosomes, and Z-disks, remains in bovine heart Purkinje fibres after extraction with Triton X-100 and low and high ionic strength solutions. Biochemically this cytoskeleton mainly consists of skeletin (Mr approximately 55 000) and ...

journal_title：European journal of cell biology

authors： Kjörell U,Thornell LE

更新日期：1982-08-01 00:00:00

abstract：:Pinocytosis was studied in cultured human skin fibroblasts in order to obtain more information about uptake in these cells of non-specific ligands with different net surface charge. The fibroblasts were obtained from a normal person and a patient homozygous for familial hypercholesterolemia. No ultrastructural differe...

journal_title：European journal of cell biology

authors： van Deurs B,Nilausen K,Faergeman O,Meinertz H

更新日期：1982-06-01 00:00:00

abstract：:A new perfusion medium for isolating cardiac myocytes from adult rats was developed, thereby yielding numerous viable cells with few morphological changes. The main factors in the isolation procedure are Ca2+ deficiency, collagenase, and mechanical dispersion. Their effects on the ultrastructure of cardiac myocytes we...

journal_title：European journal of cell biology

authors： Flegler-Balon C,Behrendt H,Gerards P,Kammermeier H

更新日期：1982-06-01 00:00:00

abstract：:The entry of sporozoites of Theileria parva into bovine lymphoid cells in vitro was studied with the electron microscope. Endocytosis is completed in less than 10 min. No local mobilization of actin or other cytoskeletal elements is detected in the cytoplasm of the cell being invaded and no engulfing pseudopods are fo...

journal_title：European journal of cell biology

authors： Fawcett DW,Doxsey S,Stagg DA,Young AS

更新日期：1982-04-01 00:00:00

abstract：:The simultaneous addition of alcian blue and tannic acid to human blood platelets during glutaraldehyde fixation leads to an excellent representation of coated membranes (CM). Coated membrane segments can be found (1) in plasmalemma, in vesicle system and lysosomal granules, or (2) as coated pits or coated vesicles. T...

journal_title：European journal of cell biology

authors： Morgenstern E

更新日期：1982-02-01 00:00:00

abstract：:Filamentous networks were isolated from rat liver and studied by electron microscopy after negative staining with uranyl acetate. Highly purified preparations of networks were used to obtain material for immunological studies, but morphological studies were made more satisfactorily on preparations purified only by suc...

journal_title：European journal of cell biology

authors： Jahn W

更新日期：1982-02-01 00:00:00

abstract：:Using an exogenous kinase, nuclear ribonucleoprotein complexes with sedimentation coefficients greater than 100S were phosphorylated in vitro before and after treatment with increasing concentrations of NaC1. The phosphorylation pattern of the proteins before raising the NaC1 concentration shows a major group of label...

journal_title：European journal of cell biology

authors： Alonso A,Fischer J,König N,Kinzel V

更新日期：1981-12-01 00:00:00

abstract：:Mouse plasma cells have been exposed to a wide range of soluble and adsorptive macromolecular tracers for 10 min to 4 h to explore the possibility of membrane recycling related to the high secretory rate of these nonregulated secretory cells. Electron microscopic examination showed in all cases labeling of multivesicu...

journal_title：European journal of cell biology

authors： Tartakoff A,Vassalli P,Montesano R

更新日期：1981-12-01 00:00:00

abstract：:Mouse pituitary neurointermediate lobes were pulse-incubated in [3H] arginine or [3H] lysine for 10 min and then chase-incubated for periods 0 to 4h. The labeled peptides from the lobes were analysed by immunoprecipitation with specific antisera, and thereafter, by acid-urea polyacrylamide gel electrophoresis. Using t...

journal_title：European journal of cell biology

authors： Loh YP,Gritsch HA

更新日期：1981-12-01 00:00:00

abstract：:Cells of Oocystis solitaria after treatment with inhibitors of a) microfibril orientation b) microfibril synthesis, and c) microfibril crystallization have been investigated with the freeze-fracturing technique. Changes in morphology of the E-face of the plasma membrane as a result of these treatments have been record...

journal_title：European journal of cell biology

authors： Robinson DG,Quader H

更新日期：1981-10-01 00:00:00

abstract：:In Physarum polycephalum (Myxomycetes) aphidicolin has been found to delay metaphase onset when applied to synchronous plasmodia 3 h before control metaphase. In contrast to the action of temperature shifts, aphidicolin treatment did not delay the initiation of the increase of thymidine kinase synthesis (EC 2.7.1.21, ...

journal_title：European journal of cell biology

authors： Eon-Gerhardt R,Tollon Y,Wright M

更新日期：1981-08-01 00:00:00

abstract：:We have coupled chlorotetracycline (CTC) fluorescence with polarized light microscopic observations of living mitotic endosperm cells of Haemanthus in order to compare the spatial distributions of Ca2+-rich endomembranes in the spindle with that of the kinetochore fibers during metaphase. In either control cells or in...

journal_title：European journal of cell biology

authors： Wolniak SM,Hepler PK,Jackson WT

更新日期：1981-08-01 00:00:00

abstract：:Pretreatment of Acanthamoeba castellanii (Neff strain) with sublytic concentrations of surface-active alkyltrimethylammonium salts (C12, C14, C16) enhanced ConA-mediated agglutination of the amoebae. Treatment with the surfactants alone did not affect the "spontaneous" agglutination of the amoebae. Electron microscopi...

journal_title：European journal of cell biology

authors： Paatero G,Isomaa B,Johansson M,Kiiskinen G

更新日期：1981-08-01 00:00:00

abstract：:The large protein bodies of the storage parenchyma cells of mung bean (Vigna radiata) cotyledons contain vesicles measuring 0.2 to 2.0 mum in diameter. The vesicles contain ribosomes, ribosomes, membranous elements which may be derived from the endoplasmic reticulum and occasionally Golgi bodies and mitochondria. The ...

journal_title：European journal of cell biology

authors： Herman EM,Baumgartner B,Chrispeels MJ

更新日期：1981-06-01 00:00:00

abstract：:The macronuclear DNA synthesis in well-fed carnivorous forms of Tetrahymena paravorax has been investigated with autoradiographic experiments. The macrostome cell type of this ciliate is induced by stomatin and fed on living prey (T. pyriformis). The DNA synthesis is re-initiated when the macrostomes become active pre...

journal_title：European journal of cell biology

authors： Méténier G

更新日期：1981-06-01 00:00:00

abstract：:The differences in the content of trace elements (Fe, Cu, Zn, Br, Rb and Sr) in phasic and tonic scallop adductor muscles have been revealed by using X-ray fluorescence analysis. The phasic (yellow) muscles have been found to be richer in Fe and Cu while more deficient in Zn and Br as compared to the tonic (white) mus...

journal_title：European journal of cell biology

authors： Ogievetskaya MM,Maslov O,Sheludko N

更新日期：1981-04-01 00:00:00

abstract：:The absence of nucleosome-like structures from purified nuclei of the primitive dinoflagellate Prorocentrum micans was demonstrated by three means. i) Electron microscopy revealed mostly thin, smooth 6-nm nucleofilaments in chromatin incubated at various ionic strengths and either fixed in 0.1% glutaraldehyde or unfix...

journal_title：European journal of cell biology

authors： Herzog M,Soyer MO

更新日期：1981-02-01 00:00:00

abstract：:Large cytoplasmic aggregates (Mallory bodies) containing randomly arranged 10 to 20 nm thick filaments are characteristic structures of hepatocytes in certain liver diseases, especially alcoholic hepatitis of man and griseofulvin intoxication of mouse. Biochemical and immunological studies have shown that such Mallory...

journal_title：European journal of cell biology

authors： Denk H,Franke WW

更新日期：1981-02-01 00:00:00

abstract：:In Euplotes crassus, the ampules which occur in groups that surround the cilia at their base are organelles which, unlike the extrusomes such as mucocysts and trichocysts, persist inside the cytoplasm and are not lost after discharge. By freeze-fracturing cells, we found membrane specializations denoted by the presenc...

journal_title：European journal of cell biology

authors： Dallai R,Luporini P

更新日期：1981-02-01 00:00:00

abstract：:High-speed centrifugation supernatants from slime mould plasmodia show considerable activities to inhibit the polymerization of actin as revealed by viscosity measurements. By following increasing inhibitory activities an actin modulating protein (AM-protein) has been isolated and purified which affects the polymer st...

journal_title：European journal of cell biology

authors： Hinssen H

更新日期：1981-02-01 00:00:00

abstract：:Coexistence of four different forms of chromatin was observed by electron microscopy in nuclear spread preparations of monkey kidney cells during late stages of infection with herpes simplex virus (HSV-1 AMG). Besides typical nucleosomal (i) chromatin, thin (3-5 nm) strands morphologically indistinguishable from prote...

journal_title：European journal of cell biology

authors： Müller U,Schröder CH,Zentgraf H,Franke WW

更新日期：1980-12-01 00:00:00

abstract：:Specific rabbit antisera were prepared against purified aggregation factor and its membrane-associated receptor, baseplate, derived from the marine sponge. Microciona prolifera. They were utilized in conjunction with fluorescent-labeled goat anti-rabbit IgG in an assay to demonstrate the surface localizations of both ...

journal_title：European journal of cell biology

authors： Kuhns WJ,Bramson S,Simpson TL,Burkart W,Jumblatt J,Burger MM

更新日期：1980-12-01 00:00:00

abstract：:1. Feeding weanling mice a diet containing 0.5% (w/w) cuprizone (biscyclohexanone oxaldihydrazone) induced megamitochondria of a different type of that previously described (Suzuki, K., Science 163, 81-82 (1969). 2. As in the normal controls, a single population of mitochondria with a narrow size distribution, was det...

journal_title：European journal of cell biology

authors： Flatmark T,Kryvi H,Tangerås A

更新日期：1980-12-01 00:00:00

abstract：:The loss of epidermal growth factor (EGF) binding activity on cultured murine 3T3 cells exposed to EGF (EGF receptor down regulation) was determined in colchicine treated cells, cytochalasin B treated cells, and untreated cells. Neither colchicine nor cytochalasin B altered the affinity of the receptor for EGF, but co...

journal_title：European journal of cell biology

authors： Wrann M,Fox CF

更新日期：1980-10-01 00:00:00